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KMID : 1377020160130050547
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Tissue Engineering and Regenerative Medicine
2016 Volume.13 No. 5 p.547 ~ p.553
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Hypoxia enhances osteogenic differentiation in retinoic acid-treated murine-induced pluripotent stem cells
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Jeeranan Manokawinchoke
Thanaphum Osathanon
Hiroshi Egusa
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Abstract
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Hypoxic condition influences biological responses in various cell types. However, a hypoxic regulating osteogenic differentiation remains controversy. Here, an influence of short-term culture in hypoxic condition on osteogenic marker gene expression by retinoic acid-treated murine gingival fibroblast-derived induced pluripotent stem cells (RA-miPS) was investigated. Results demonstrated that hypoxic condition significantly upregulated Vegf, Runx2, Osx, and Ocn mRNA expression by RA-miPS in normal culture medium at day 3. Further, desferrioxamine significantly downregulated pluripotent marker (Nanog and Oct4) and enhanced osteogenic marker (Runx2, Osx, Dlx5, and Ocn) gene expression as well as promoted in vitro mineral deposition. However, the effect of cobalt chloride on osteogenic differentiation of RA-miPS was not robust. In summary, the results imply that hypoxic condition may be useful in the enhancement of osteogenic differentiation in RA-miPS.
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KEYWORD
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Pluripotent stem cells, Hypoxia, Desferrioxamine, Cobalt chloride
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